James Hadfield - Core Facility Manager
Talk to me about: All things NGS, Microarray, Library preparation, qPCR, Sanger sequencing, experimental design and technology development.
- James is a molecular biologist and runs the genomics core facility at the University of Cambridge, Cancer Research UK Cambridge Institute. He has many years experience in genomic technologies including NGS and microarrays. James thinks genomics is the next best thing since sliced bread.
- James received a Biology degree from the University of East Anglia in 1995. His career so far has leant towards technology development or implementation. In 1995 he developed a differential PCR test for ErbB2 copy number analysis at the same time as real-time PCR came along, a poor choice in hindsight!
Over the last 16 years he's worked at; the Norfolk & Norwich Hospital on ErbB2, Royal London on Diabetes genetics, the Cambridge Uni Department of Pathology on Immunology group and the John Innes Centre on Wheat disease resistance gene cloning and arrays. In 2000 he set up an Affy and spotted microarray facility at JIC, he co-founded the UK Affy user group, which is still going strong. At JIC he also won a Biotech competition (doesn't he look young!), and hopes one-day to start a business - although none of his ideas have come to anything yet!
In 2006 James moved to set up the genomics facility at CRI. The lab offers broad spectrum genomic services for scientists at CRI and Illumina next-gen sequencing for CRI, the Gurdon Institute, the MRC-LMB, the Stem Cell Institute, the Institute of Metabolic Sciences, the Cambridge Molecular Diagnostics Laboratory and the departments of Haematology, Obs & Gynae and Medical genetics .
James has two kids and lives in Norfolk, which is a lot nearer the coast than Cambridge.
Publications by James Hadfield:
Hannah Haydon - Senior Scientific Associate
Hannah received her BSc in Biological Sciences specialising in Molecular Genetics at Warwick University (2006). After graduating, she began her career as a Contract Research Scientist for a genomics service provider. Gaining experience in SNP genotyping and Sanger sequencing and project manager of microarrays and real time PCR projects. She moved to the Wellcome Trust Sanger Institute as a Research Assistant in tissue culture before moving onto CRUK. In genomics, Hannah was one of the core members of the sequencing team involved in Illumina next-generation sequencing and library preparation. She was recently promoted to manage the library prep services in the Genomics Core Facility - taking over from Michelle.
Fatimah Bowater - Senior Scientific Associate
Fatimah graduated from Sheffield Hallam University 2007 in BSc (Hons) Cellular and Molecular Biosciences. She previously worked for Source BioSciences in their sequencing services before moving to the Cambridge Institute in 2008. She has gained experience in Sanger sequencing, Illumina next-generation sequencing and library preparation. In genomics, Fatimah was one of the core members of the sequencing team involved in Illumina next-generation sequencing and library preparation. She was recently promoted to manage the sequencing services in the Genomics Core Facility - taking over from Sarah.
Marta Grzelak - Senior Scientific Associate
Marta currently runs the Core's library prep services. She moved from Poland where she obtained a MSc degree in biotechnology and worked at the University of Warsaw gaining experience in various library preparation methods and Illumina next-generation sequencing as well as basic sequencing data analysis.
Rosalind Launchbury - Scientific Officer
Rosalind graduated in 1995 with a BSc (Hons) in Human biology from Oxford Brookes University. She then went on to do an MSc in Reproductive Biology at Edinburgh University and worked in research labs at the Western General Hospital. Rosalind then moved to Oxford where she worked in oncology at the John Radcliffe Hospital. The final move was to Cambridge where she worked as a Scientific Officer in Jason Carroll’s lab at CI before taking a post in the Genomics Core. She has extensive experience of ChIP-seq, Illumina next-generation sequencing and library preparation. She is a Scientific Officer II in the Genomics Core Facility.
Johanna Barbieri - Scientific Officer
Johanna has come from France to sunny Cambridge. She did her Undergraduate degree on Reunion island studying Microbiology. She followed this up with a Master's degree in Molecular Diagnostics from Toulouse University. Following this, Johanna went off to work for the French National Institute for Agronomic Research (INRA) in Toulouse, working in the sequencing facility PlaGe.
She has extensive experience Illumina next-generation sequencing and library preparation.
Tom Denyer - Scientific Officer
Tom joined us in June 2015. He graduated in 2008 with a BSc (hons) in Microbiology from Nottingham Trent University. This was followed by an MRes in Genomic and Proteomic Sciences and a PhD looking into microRNAs in Arabidopsis roots; both undertaken at the University of Nottingham. Between the Masters degree and PhD, Tom worked for TGAC in Norwich as a Research Assistant in the sequencing library construction group. He is now a Scientific Officer (or Research assistant) in the Genomics Core Facility.
Previous staff members:
Sarah Leigh-Brown - now working at AbCam
Michelle Pugh - now working at Inivata
Claire Fielding - now working at Illumina in R&D
Sarah Aldridge - now working in Astra Zeneca's companion diagnostics division
Sara Sayalero - now working in Barcelona
Christian Bourne - now working at Illumina
Inma Spiteri - now in the Sottoriva lab at ICR
Dr Jessica Davies - now working in Oxford
Nik Matthews - now heading the Cancer Research UK London Research Institute's NGS labs, and shortly moving to head the genomics facility at the CRICKstitute.