skip to primary navigationskip to content
 

Equipment and other services

Equipment in the CIGC

Here is a full list of equipment we have in the Genomics Core (some of which is restricted access). If you need training or want to access the equipment please email the .

Book time on the machines using Resource Scheduler.

  • 2x Illumina HiSeq 4000
  • 1x Illumina HiSeq 2500
  • 2x Illumina MiSeq
  • 1x Illumina NextSeq
  • 10X Genomics Chromium
  • Fluidigm BioMark
  • 2x Fluidigm AccessArray system
  • 2x QuantStudios for real-time PCR
  • 2x Qiagen QIAcube for automating Qiagen column extractions
  • Agilent Bravo Automated Liquid Handling robot
  • 2x Agilent BioAnalyser
  • Qubit
  • 1x Covaris S220 DNA fragmentation system
  • 1X Covaris LE220 DNA fragmentation system

Nucleic Acid QC with the Agilent Bioanalyser

We have two Agilent BioAnalysers for quantity assessment of RNAs and also for Illumina NGS libraries. We hold kits in stock for you to use for the common applications and you need to sign-out each chip you use. There is a lot of experience in the core using this instrument so if you’d like some help just ask.

Here are some easy guide to using the Agilent Bioanalyser: Agilent RNA 6000 Nano Users Guide and Agilent DNA 1000 users guide.

Automated Nucleic Acid Extraction

We have two Qiacubes for nucleic acid extraction, which automate sample preparation using Qiagen spin-column kits. Before using the Qiacube it is very important to note that each protocol requires the use of different tube types for sample processing and collection, see below for details.

Purchase your own reagents:

RNA: We recommend either miRNAeasy or All-Prep for extraction as either will allow analysis of both microRNAs (small RNA species) and mRNAs (large RNA species).

DNA: We recommend DNAeasy blood and QIAamp circulating nucleic acid kits for ctDNA.