The Fluidigm BioMark:
The BioMark is a highly parallelized and miniaturised real-time PCR instrument allowing you to run 1000’s or even 10,000’s of reactions per day in microfludic chips. The system has been used primarily for digital-PCR and AccessArray amplification of loci for Illumina sequencing. It can also be used for TaqMan and SYBR-based gene expression and genotyping analysis. Microfluidic- chips come in 48x48 and 96x96 format combining either 48 or 96 samples and assays allowing ~2,500 or ~10,000 reactions per run. It uses around 5ul of sample/assay per run by performing nanolitre reactions, this may require a pre-amplification step which would not be used in a standard qPCR experiment.
The Fluidigm AccessArray:
The Access Array uses the same microfluidic technology as the Biomark and allows 48 DNA samples to be PCR amplified for 48 loci (or 48 pools of <20 loci) whcich can then be seqeunced directly on the HiSeq or MISeq.
The Fluidigm C1:
The C1 Single-Cell Auto Prep System uses the same microfluidic technology as the Biomark and allows up to 96 single cells to be captured from a cell suspension. You can profile unique genomes and transcriptomes. The C1 can extract, reverse transcribe and amplify cDNA ready for Nextera-based libarry prep and HiSeq seqeuncing.
Training and access:
Simply book training and time on the machines to use them. The training does cost around £2000 each time for up to 5 people so the CIGC will help co-ordinate this with other interested users.
Buying chips to run:
Purchase your own chips and reagents for gene-expression, genotyping and digital PCR, the CIGC holds a small stock of chips and reagents for AccessArray amplification.